Continuing their quest to find the lost, enchanted manuscript named Ashmole 782, witch-in-training Diana Bishop and her lover, vampire geneticist Matthew Clairmont, travel back in time to the Elizabethan London, where they meet up with some of Matthew's old vampire colleagues in the School of Night. More people from Matthew's past show up than intended, however, including some old enemies, and now the fate of Ashmole 782 is in jeopardy.